Protease production by soil bacteria for green technology: Screening and optimization

Abstract Protease is a type of enzyme that hydrolyzes proteins into peptides and amino acids. The demand for protease green technology in some industries like detergent, food, leather increasing nowadays. Some soil bacteria, especially the Bacillus strains, are known to have ability produce protease. Compared other sources plant animal origin, microbial has more advantages as it can be produced at an industrial scale, short cultivation time, easy harvest. This research aimed screen proteolytic bacteria from bulk samples collected under mango trees study optimum condition production using response surface methodology (RSM). Three bacterial strains (SH2CR, SH3CR, SC4CR) were isolated shown activity. Based on 16S rRNA gene analysis, strain SH2CR was close Priestia megaterium NBRC 15308 T (96.97% similarity), while SH3CR SC4CR related zanthoxyli 1433 (100% similarity). One them, SH2CR, further studied fermenter one-liter scale with medium containing skim milk 30°C. best by achieved 48 h incubation 300 rpm agitation, 1.25% milk.